Reverse transcription is a process where RNA is converted into cDNA using an enzyme called reverse transcriptase. This enzyme synthesizes a complementary DNA strand based on an RNA template. HiScript II Reverse Transcriptase is a highly efficient and thermostable enzyme that exhibits enhanced activity, specificity, and robustness compared to its predecessor.

Some key features and advantages of HiScript II Reverse Transcriptase include:

1. High Efficiency: HiScript II Reverse Transcriptase offers high sensitivity and strong reverse transcription capability, allowing efficient conversion of RNA to cDNA.
   
   
2. Wide Dynamic Range: It can handle a broad range of RNA concentrations, making it suitable for various applications, including both low and high abundance RNA samples.
   
   
3. Thermostability: HiScript II Reverse Transcriptase is stable at elevated temperatures, allowing it to withstand higher reaction temperatures and reduce the risk of RNA degradation.
   
   
4. Enhanced Sensitivity: The enzyme exhibits improved sensitivity to low copy RNA targets, enabling reliable detection and quantification of rare transcripts.
   
   
5. Broad Compatibility: HiScript II Reverse Transcriptase is compatible with various RNA templates, including total RNA, mRNA, and viral RNA.
   
   
6. Reduced RNase H Activity: The enzyme exhibits reduced RNase H activity, minimizing degradation of RNA during the reverse transcription process.
   
   
7. Convenience: HiScript II Reverse Transcriptase is easy to use and compatible with standard reverse transcription protocols. It is commonly available as a ready-to-use enzyme mix, simplifying experimental setup.